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optical flat pcr tube 8 cap strips  (Bio-Rad)


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    Structured Review

    Bio-Rad optical flat pcr tube 8 cap strips
    Optical Flat Pcr Tube 8 Cap Strips, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optical+flat+pcr+tube+8+cap+strips/Flat+PCR+Tube+8-Cap+Strips/pmc11993131-89-50-63
    Average 99 stars, based on 76 article reviews
    optical flat pcr tube 8 cap strips - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Characterization of Anaphylatoxin Receptor Expression and C3a/C5a Functions in Anaphylatoxin Receptor Reporter Mice.
    Article Snippet: Materials Floxed GFP-C5aR1, floxed tdTomato-C5aR2 or tdTomato-C3aR knockin mice (Köhl laboratory) Thioglycolate-elicited peritoneal macrophages (see Current Protocols article: Zhang et al., 2008) DMEM medium (Thermo Fisher Scientific Gibco, cat. no. 31885-023) Dulbecco’s phosphate-buffered saline (D-PBS) without calcium or magnesium, pH 7.0-7.3 (Thermo Fisher Scientific, cat. no. 14190144) RNAeasy Mini Kit (Qiagen, cat. no. 74104) Nuclease-free water (Thermo Fisher Scientific, cat. no. R0581) DNase I buffer (10×) with Mg2+: 100 mM Tris·HCl (pH 7.5 at 25°C)/25 mM MgCl2/1 mM CaCl2 (part of the DNase I kit; Thermo Fisher Scientific, cat. no. EN0525) DNase I (Thermo Fisher Scientific, cat. no. EN0525) 50 mM EDTA (part of DNase I kit; Thermo Fisher Scientific, cat. no. EN0525) Oligo(dT)18 primer (Thermo Fisher Scientific, cat. no. S0132) 10× dNTP (Thermo Fisher Scientific, cat. no. R0191) RNaseOUT recombinant RNase inhibitor (Thermo Fisher Scientific, cat. no. 10777019) 5× RT buffer: 250 mM Tris·HCl (pH 8.3 at 25°C)/250 mM KCl/20 mM MgCl2/50 mM DTT (part of the RevertAid kit; Thermo Fisher Scientific, cat. no. EP0441) RevertAid reverse transcriptase (Thermo Fisher Scientific, cat. no. EP0441) Primers for C5ar1, C5ar2, C3ar1 (100 μM; Eurofins Genomics or Thermo Fisher Scientific) iQ SYBR Green Supermix (BioRad, cat. no. 1708880) 1.5% TBE agarose gel (see Current Protocols article: Voytas, 2001) O’Gene Ruler Ladder, 50 bp (Thermo Fisher Scientific, cat. no. SM 0371) 24-well plates Cell scraper (Sarstedt, cat. no. 83.1830) 1.5-ml microcentrifuge tubes (Sarstedt, cat. no. 72.690.001), autoclaved Refrigerated centrifuge NanoDrop Microvolume Spectrophotometer (Thermo Fisher Scientific, cat. no. ND-2000) Two heat blocks 0.2-ml 8-microtube strip without caps: high-profile (BioRad, cat. no. TBS0201) or low-profile (BioRad, cat. no. TLS0801) depending on your thermocyclerLaumonnier et al. 20 of 41 Current Protocols in Immunology 0.2-ml optical flat PCR-tube 8-cap strips, ultraclear (Bio-Rad, cat. no. TCS0803) 1.5-ml screw-cap microcentrifuge tubes with O-rings (Sarstedt, cat. no. 72.692.405) Thermal cycler Additional reagents and equipment for anesthesia (see Current Protocols article: Donovan & Brown, 2001) and euthanasia (see Current Protocols article: Donovan & Brown, 2006) of mice, counting cells (see Current Protocols article: Strober, 2001), and agarose gel electrophoresis (see Current Protocols article: Voytas, 2001) 1.

    Polymerase Chain Reaction:

    Article Title: Prolyl oligopeptidase acts as a link between chaperone-mediated autophagy and macroautophagy.
    Article Snippet: Antibody Species Manufacturer Product# Dilution WB IHC LAMP-2A Rabbit Abcam ab18528 1/1000 1/200 Hsc70 Rat Abcam ab51052 1/5000 – GAPDH Mouse Abcam ab8245 1/5000 – LC3B Rabbit Sigma L7543 1/1000 – α-Synuclein Mouse Abcam ab80627 1/1000 – Vinculin Rabbit Abcam ab129002 1/10000 – Cadherin Mouse Abcam ab1416 – 1/200 H. Cui et al. Biochemical Pharmacology 197 (2022) 114899 65-95°C for melt-curve analysis). .. White 0.2 ml 8-Tube PCR Strips (#TLS0851; Bio-Rad) and optical flat PCR Tube 8-Cap Strips (#TCS0803; Bio-Rad) were used for qPCR. ..

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNase ZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy.
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNaseZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Prolyl oligopeptidase acts as a link between chaperone-mediated autophagy and macroautophagy.
    Article Snippet: Antibody Species Manufacturer Product# Dilution WB IHC LAMP-2A Rabbit Abcam ab18528 1/1000 1/200 Hsc70 Rat Abcam ab51052 1/5000 – GAPDH Mouse Abcam ab8245 1/5000 – LC3B Rabbit Sigma L7543 1/1000 – α-Synuclein Mouse Abcam ab80627 1/1000 – Vinculin Rabbit Abcam ab129002 1/10000 – Cadherin Mouse Abcam ab1416 – 1/200 H. Cui et al. Biochemical Pharmacology 197 (2022) 114899 65-95°C for melt-curve analysis). .. White 0.2 ml 8-Tube PCR Strips (#TLS0851; Bio-Rad) and optical flat PCR Tube 8-Cap Strips (#TCS0803; Bio-Rad) were used for qPCR. ..

    Reverse Transcription:

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNase ZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy.
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNaseZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    SYBR Green Assay:

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNase ZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy.
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNaseZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Lysis:

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNase ZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Article Title: Iron-Sulfur Clusters and Iron Responsive Element Binding Proteins Mediate Iron Accumulation in Corneal Endothelial Cells in Fuchs Dystrophy.
    Article Snippet: .. The following materials and reagents were used: Invitrogen TRIzol Reagent, UltraPure water, InvitrogenRNaseZAP RNase decontamination solution (Invitrogen, Carlsbad, CA, USA), and Applied Biosystems High Capacity cDNA reverse transcription kit (Life Technologies-Thermo Fisher Scientific, Waltham, MA, USA); RNeasy Micro Kit (QIAGEN, Germantown, MD, USA); and, Multiplate white 96-well PCR plates, optical flat PCR tube 8-cap strips, and SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA); and RIPA buffer detergent supplemented with protease inhibitors (1 Roche EDTA-free P.I.C. tablet in 10 mL lysis buffer + 1mM PMSF + 1mM vanadate). .. Primer sequences were designed with open-source Primer3 or Primer3 Plus software and were synthesized by Integrated DNA Technologies (Coralville, IA, USA).



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Neural circuit-specific gene manipulation in mouse brain in vivo using split-intein-mediated split-Cre system

    doi: 10.1016/j.xpro.2022.101807

    Figure Lengend Snippet:

    Article Snippet: 0.2 mL Flat PCR Tube 8-Cap Strips optical ultraclear , Bio-Rad , TLS0803.

    Techniques: Virus, Recombinant, Injection, Electron Microscopy, Plasmid Preparation, Gel Extraction, Software, Microscopy, Real-time Polymerase Chain Reaction, Hood, Imaging